hs68 human dermal fibroblast cell line  (ATCC)

Journal: Antioxidants

Article Title: 10(E)-Pentadecenoic Acid Inhibits Melanogenesis Partly Through Suppressing the Intracellular MITF/Tyrosinase Axis

doi: 10.3390/antiox13121547

Figure Lengend Snippet: Effects of 10E-PDA on inhibiting melanogenesis in α-MSH-stimulated B16F10 melanoma cells. ( a ) B16F10, ( b ) HS68, and ( c ) HaCaT cells were treated with increasing concentrations of 10E-PDA (1–100 μM) for 24 h, after which cell viability was assessed (n = 4 per group). ( d , e ) B16F10 cells were pretreated with different concentrations of 10E-PDA (1–15 μM) or kojic acid (30 μM) for 1 h, followed by exposure to α-MSH (500 nM) for 6 days to measure melanin content ( d ) or for 3 days to examine tyrosinase activity ( e ). In both panels, the white bar represents the untreated control group without α-MSH stimulation, the black bar represents the α-MSH-stimulated control group, and the dark gray bars indicate the 10E-PDA-treated groups under α-MSH stimulation. In panel ( e ), the light gray bar represents the positive control group treated with kojic acid under α-MSH stimulation. Data are shown as mean ± SEM. ### p < 0.001 compared with the untreated control group, and * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the α-MSH-treated group.

Article Snippet: The B16F10 murine melanoma cell line and Hs68 human dermal fibroblast cell line were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Activity Assay, Control, Positive Control

Journal: Molecular Medicine Reports

Article Title: Improvement of wound healing by capsaicin through suppression of the inflammatory response and amelioration of the repair process

doi: 10.3892/mmr.2023.13042

Figure Lengend Snippet: Changes in Hs68 cell characteristics in the presence of CAP. Hs68 cell characteristics were examined following treatment of cells with different CAP concentrations (A) Cell viability, (B) migration (cells were also treated with MMC and TNF-α) and (C) percentage closure based on the cell migration assay were determined. (D) Relative mRNA levels of IL-6 in Hs68 cells treated with TNF-α and CAP. Scale bar, 500 µm. **P<0.01, ****P<0.0001. CAP, capsaicin; IL-6, interleukin 6; MMC, mitomycin C; TNF-α, tumor necrosis factor-α.

Article Snippet: The human diploid dermal fibroblast Hs68 cell line (passage 19), which is frequently used in dermal research and has the characteristics of primary dermal fibroblasts , was purchased from the Bioresource Collection and Research Center in the Food Industry Research and Development Institute (Hsinchu, Taiwan).

Techniques: Migration, Cell Migration Assay

Journal: Antioxidants

Article Title: Pholiota nameko Polysaccharides Protect against Ultraviolet A-Induced Photoaging by Regulating Matrix Metalloproteinases in Human Dermal Fibroblasts

doi: 10.3390/antiox11040739

Figure Lengend Snippet: Cell viability analyzed via MTT assay. Cell viability was assessed using 3-(4,5-dimethylthiazolyl-2) 2,5 diphenyltetrazolium bromide (MTT) assays. Hs68 cells were treated with PNPs for 24 h. Experiments were conducted in triplicate ( n = 3). Data are expressed as the mean ± SD. * Significant differences compared to the control group ( p < 0.05). a,b: Matching lowercase letters between samples at the same concentration indicate significant differences between the samples at that concentration ( p < 0.05). A–C: Matching uppercase letters for the same sample at different concentrations indicate significant differences for that sample between different concentrations ( p < 0.05).

Article Snippet: Human dermal fibroblast cell line Hs68 (obtained from ATCC CRL-1635, Manassas, VA, USA) was cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco ® , Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco ® , Grand Island, NY, USA) and antibiotics (100 U penicillin and 100 U/mL streptomycin; Gibco ® , Grand Island, NY, USA) under 5% CO 2 at 37 °C.

Techniques: MTT Assay, Control, Concentration Assay

Journal: Antioxidants

Article Title: Pholiota nameko Polysaccharides Protect against Ultraviolet A-Induced Photoaging by Regulating Matrix Metalloproteinases in Human Dermal Fibroblasts

doi: 10.3390/antiox11040739

Figure Lengend Snippet: Cell viability of Hs68 cells pretreated with PNPs (62.5, 125, 250, and 500 μg/mL) for 24 h and then subjected to UVA irradiation at 5 J/cm 2 , as determined via MTT assay. Experiments were conducted in triplicate ( n = 3). Data are expressed as the mean ± SD. * Significant differences compared to the UVA-induced group ( p < 0.05). a–c: Matching lowercase letters between samples at the same concentration indicate significant differences between the samples at that concentration ( p < 0.05). A,B: Matching uppercase letters for the same sample at different concentrations indicate significant differences for that sample between different concentrations ( p < 0.05).

Article Snippet: Human dermal fibroblast cell line Hs68 (obtained from ATCC CRL-1635, Manassas, VA, USA) was cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco ® , Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco ® , Grand Island, NY, USA) and antibiotics (100 U penicillin and 100 U/mL streptomycin; Gibco ® , Grand Island, NY, USA) under 5% CO 2 at 37 °C.

Techniques: Irradiation, MTT Assay, Concentration Assay

Journal: Antioxidants

Article Title: Pholiota nameko Polysaccharides Protect against Ultraviolet A-Induced Photoaging by Regulating Matrix Metalloproteinases in Human Dermal Fibroblasts

doi: 10.3390/antiox11040739

Figure Lengend Snippet: ( a ) Radical scavenging activity of PNPs (250 µg/mL) on Hs68 cells against UVA-induced ROS generation (scale bar = 100 µm, magnification: 10 × 10). ( b ) Quantitative analysis (performed using Image J software) of the radical scavenging effect of PNPs on Hs68 cells against UVA-induced ROS generation. Experiments were independently conducted in triplicate ( n = 3). Data are expressed as the mean ± SD. a–c: Matching lowercase symbols indicate significant differences between the different groups ( p < 0.05).

Article Snippet: Human dermal fibroblast cell line Hs68 (obtained from ATCC CRL-1635, Manassas, VA, USA) was cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco ® , Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco ® , Grand Island, NY, USA) and antibiotics (100 U penicillin and 100 U/mL streptomycin; Gibco ® , Grand Island, NY, USA) under 5% CO 2 at 37 °C.

Techniques: Activity Assay, Software

Journal: Antioxidants

Article Title: Pholiota nameko Polysaccharides Protect against Ultraviolet A-Induced Photoaging by Regulating Matrix Metalloproteinases in Human Dermal Fibroblasts

doi: 10.3390/antiox11040739

Figure Lengend Snippet: Protective effect of PNPs against cellular senescence in Hs68 cells irradiated with UVA. Experiments were conducted in triplicate ( n = 3). Data are expressed as the mean ± SD. a–c: Matching lowercase letters indicate significant difference between the different groups ( p < 0.05). Experiments were conducted in triplicate ( n = 3). Data are expressed as the mean ± SD.

Article Snippet: Human dermal fibroblast cell line Hs68 (obtained from ATCC CRL-1635, Manassas, VA, USA) was cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco ® , Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco ® , Grand Island, NY, USA) and antibiotics (100 U penicillin and 100 U/mL streptomycin; Gibco ® , Grand Island, NY, USA) under 5% CO 2 at 37 °C.

Techniques: Irradiation

Journal: Antioxidants

Article Title: Pholiota nameko Polysaccharides Protect against Ultraviolet A-Induced Photoaging by Regulating Matrix Metalloproteinases in Human Dermal Fibroblasts

doi: 10.3390/antiox11040739

Figure Lengend Snippet: Protective effect of PNPs against UVA irradiation in Hs68 cells. Protein expression of MMP-1, -3, and -9 was analyzed via Western blot. ( a ) Hs68 cells were pretreated with PNPs (250 µg/mL) and RA (10 µM) for 24 h, followed by UVA-irradiation. Western blotting was performed to examine MMP-1, -3 and -9 expression, with GAPDH used as an internal control. ( b – d ) MMP-1, -3, and -9 expression after quantification using Image J software. a–e: Matching lowercase letters indicate significant differences between the different groups ( p < 0.05). Experiments were conducted in triplicate ( n = 3), and the blot shown was the representative result. Data are expressed as the mean ± SD.

Article Snippet: Human dermal fibroblast cell line Hs68 (obtained from ATCC CRL-1635, Manassas, VA, USA) was cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco ® , Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco ® , Grand Island, NY, USA) and antibiotics (100 U penicillin and 100 U/mL streptomycin; Gibco ® , Grand Island, NY, USA) under 5% CO 2 at 37 °C.

Techniques: Irradiation, Expressing, Western Blot, Control, Software

Journal: Antioxidants

Article Title: Functional Analysis of Macromolecular Polysaccharides: Whitening, Moisturizing, Anti-Oxidant, and Cell Proliferation

doi: 10.3390/antiox8110533

Figure Lengend Snippet: The 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) assay results showing that A. fulica extracts and H. retispora extracts treatment decreased ROS production in HS68 cells. The phorbol-12-myristate-13-acetate (PMA) was used as negative control to increase the oxidative level. Data represents mean ± S.D of three independent experiments performed. (Data represents mean ± S.D of three independent experiments performed. * p < 0.01).

Article Snippet: A total of 5% CO 2 at 37 °C was used to cultivate human dermal fibroblasts cell line HS68 (ATCC ® CRL-1635TM) (ATCC, Manassas, VA, USA) cell at a consistent monolayer culture of Dulbecco’s modified Eagle medium (DMEM) for 24 h. Fetal bovine serum (FBS) (10%), penicillin (100 U/mL), streptomycin (100 mg/mL), amphotericin B (0.25 μg/mL), and amphotericin B (0.25 μg/mL) were the ingredients of DMEM.

Techniques: Negative Control

Journal: Antioxidants

Article Title: Functional Analysis of Macromolecular Polysaccharides: Whitening, Moisturizing, Anti-Oxidant, and Cell Proliferation

doi: 10.3390/antiox8110533

Figure Lengend Snippet: (A) HS68 cells collagen production with A. fulica extracts and H. retispora extracts treatments in Sirius red assay. PMA is used as negative control. ( B ) The quantitative data of collagen production; Data represents mean ± S.D of three independent experiments performed. (Data represents mean ± S.D of three independent experiments performed. * p < 0.01).

Article Snippet: A total of 5% CO 2 at 37 °C was used to cultivate human dermal fibroblasts cell line HS68 (ATCC ® CRL-1635TM) (ATCC, Manassas, VA, USA) cell at a consistent monolayer culture of Dulbecco’s modified Eagle medium (DMEM) for 24 h. Fetal bovine serum (FBS) (10%), penicillin (100 U/mL), streptomycin (100 mg/mL), amphotericin B (0.25 μg/mL), and amphotericin B (0.25 μg/mL) were the ingredients of DMEM.

Techniques: Negative Control